English
norskBlar i forfatter "Sanabria-Moreno, Adriana Maria"
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Culturing periprosthetic tissue in BacT/Alert® Virtuo blood culture system leads to improved and faster detection of prosthetic joint infections
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-07-10)<i>Background</i> - Blood culture bottles (BCBs) provide a semiautomated method for culturing periprosthetic tissue specimens. A study evaluating BCBs for culturing clinical samples other than body fluids is needed before implementation into clinical practice. Our objective was to evaluate use of the BacT/Alert® Virtuo blood culture system for culturing periprosthetic tissue specimens. <p> ... -
A Shotgun-metagenomics approach for laboratory diagnostics in clinical microbiology
(Doctoral thesis; Doktorgradsavhandling, 2020-11-12)Shotgun-metagenomics (SMg) has a huge potential, particularly in clinical microbiology where conventional diagnostic methods have limitations such as diagnosis of prosthetic joint infection (PJI). The main objective of this project was to explore the use of SMg in clinical diagnostics of PJI directly from blood culture bottles (BCBs) inoculated with PJT specimens. First, we assessed the use of a ... -
Shotgun-metagenomics based prediction of antibiotic resistance and virulence determinants in Staphylococcus aureus from periprosthetic tissue on blood culture bottles
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-10-21)Shotgun-metagenomics may give valuable clinical information beyond the detection of potential pathogen(s). Identification of antimicrobial resistance (AMR), virulence genes and typing directly from clinical samples has been limited due to challenges arising from incomplete genome coverage. We assessed the performance of shotgun-metagenomics on positive blood culture bottles (n = 19) with periprosthetic ... -
Shotgun-Metagenomics on Positive Blood Culture Bottles Inoculated With Prosthetic Joint Tissue: A Proof of Concept Study
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-07-17)Clinical metagenomics is actively moving from research to clinical laboratories. It has the potential to change the microbial diagnosis of infectious diseases, especially when detection and identification of pathogens can be challenging, such as in prosthetic joint infection (PJI). The application of metagenomic sequencing to periprosthetic joint tissue (PJT) specimens is often challenged by low ...
